Parents that are homozygous for different mutations a, b and c are crossed, producing offspring that are heterozygous as following

aa X bb        =  ab  (X)          

 bb X cc         =  bc  (Y)

aa X cc          =   ac  (Z)

If a and b belong to same locus but b and c belong to different locus then phenotype of heterozygote

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#Question id: 23251

#SCPH28 | Zoology

Which enzyme degrades the resulting component dNTPs and ATP if not incorporated in chain.

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#Question id: 745

#SCPH05 I Biotechnology

The structure of RNA differs from that of DNA, as RNA contains:

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#Question id: 7761

#SCPH28 | Zoology

Correct sets of genes are well known to be involved in aging and its prevention, and each set appears to be conserved between phyla and even kingdoms. 

a. DNA repair enzymes

b. proteins of the insulin signaling pathway

c. proteins in the mTORC1 signaling pathway

d. chromatin remodeling enzymes

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#Question id: 15618

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?

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#Question id: 13055

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?