During glycolysis, glucose 1-phosphate is converted to fructose 6-phosphate in two successive reactions: Glucose 1-phosphate -> glucose 6-phosphate DG'° = –7.1 kJ/mol Glucose 6-phosphate -> fructose 6-phosphate DG'° = +1.7 kJ/mol DG'° for the overall reaction is:

TLS Online TPP Program

#Question id: 5243

#SCPH28 | Zoology

Auxotroph culture (His- and Lac-) culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select prototroph for both His+  and Lac+ cells?

TLS Online TPP Program

#Question id: 13081

#SCPH28 | Zoology

Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

TLS Online TPP Program

#Question id: 9581

#SCPH05 I Biotechnology

These four Photosystem II, Cytochrome b6 f, Photosystem I and ATP synthase integral membrane complexes are vectorially oriented in the thylakoid membrane to function as follows, find out the incorrect statements;

TLS Online TPP Program

#Question id: 12714

#SCPH06 I Botany

Physiological and biochemical perturbations in plants caused by fluctuations in the abiotic environment, given column I with environmental factor and with its effects in the form of primary effects  in column II and secondary effects  in column III

COLUMN I        (environmental factor )	COLUMN II            (primary effects)	COLUMN III         (secondary effects)
A) Water deficit	i) Hypoxia,  Anoxia	a) Membrane dysfunction
B) High temperature	ii) ROS production	b) Reduced cellular and metabolic activities, Leaf abscission, Ion cytotoxicity and Cavitation
C) Trace element toxicity	iii) Photo inhibition	c) Reduced respiration,     Fermentative metabolism,       Inadequate ATP production ,  ROS production and  Stomatal closure
D) Flooding and soil compaction	iv) Membrane destabilization	d) Inhibition of PSII repair
E) Chilling	v) Cell dehydration and Hydraulic resistance	e) Disruption of metabolism
F) High light intensity		f) Photosynthetic and respiratory inhibition, ROS production

Find out the correct combination of given above column;

TLS Online TPP Program

#Question id: 19306

#SCPH28 | Zoology

35S promoter sequence is derived from which plant virus