A sodium-potassium pump: -triyambak.org

Practice Questions

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#Question id: 2119

A sodium-potassium pump:

#SCPH06 I Botany

moves three potassium ions out of a cell and two sodium ions into a cell while producing ATP for each cycle
moves three sodium ions out of a cell and two potassium ions into a cell using energy from ATP hydrolysis
moves three potassium ions out of a cell and two sodium ions into a cell using energy from ATP hydrolysis
move three sodium ions out of a cell and two potassium ions into a cell and generates an ATP in each cycle

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#Question id: 634

#SCPH05 I Biotechnology

An enzyme has a Vmax of 50 micromol product formed (minute x mg protein)-1 and a Km of 10 microM for the substrate. When a reaction mixture contains the enzyme and 5 microM substrate, which of the following percentages of the maximum velocity will be closest to the initial reaction rate?

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#Question id: 5122

#SCPH01 Biochemistry

In amphibia, when epidermal (ectodermal) and mesodermal cells are brought together in a mixed aggregate, the epidermal cells move to the periphery of the aggregate, and the mesodermal cells move to the inside by the process of selective affinity. Which of the following statement describe the phenomena of selective affinity?

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#Question id: 17661

#SCPH05 I Biotechnology

If the viscosity of a fluid increases with increasing stirrer speed, then the nature of the fluid is

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#Question id: 13091

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.