Practice Questions

TLS Online TPP Program

#Question id: 3680

If DNA polymerase I were mutated so that all its enzymatic activities were inactive, which part of replication would be most affected?

#SCPH06 I Botany

synthesis of Okazaki pieces
joining of Okazaki pieces
proof-reading of DNA
adjusting of supercoiling

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TLS Online TPP Program

#Question id: 11798

#SCPH05 I Biotechnology

Companion cells transport of photosynthetic products from producing cells in mature leaves to the sieve elements in the minor veins of the leaf. There are at least three different types of companion cells in the minor veins of mature, exporting leaves, All three cell types have dense cytoplasm and abundant mitochondria;

TLS Online TPP Program

#Question id: 18633

#SCPH01 Biochemistry

Phylogenetic tree provides information about

TLS Online TPP Program

#Question id: 13090

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.