Practice Questions

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#Question id: 4165

Which is true about proofreading activity during the synthesis of aminoacyl-tRNAs?

#SCPH06 I Botany

Proofreading is accomplished as an amino acid enters the active site of the enzyme. Incorrect amino acids are rejected before any reaction occurs.
Proofreading occurs just after aminoacyl-adenylate formation.
A separate cytosolic proofreading enzyme catalyzes the hydrolysis of incorrect aminoacyl-tRNAs.
No proofreading activity is necessary due to the high level of specificity of all aminoacyl-tRNA synthetases.

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#Question id: 5252

#SCPH28 | Zoology

Which type(s) of genetic transfer lead to incorporation of new DNA into the bacterial chromosome?

I. Conjugation mediated by F factor

II. Hfr mediated conjugation

III. Transduction

IV. Transformation

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#Question id: 12911

#I Life Science/ Life Sciences Group – I-V

Match the following:

A. DNA polymerases i. Alkaline phosphatase a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase ii. T4 DNA ligase b. Thermostable DNA polymerase used in PCR

C. Ligases iii. AMV-RT c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases iv. Taq DNA pol d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

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#Question id: 16001

#SCPH05 I Biotechnology

Disaggregating of cells can be achieved by

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#Question id: 19351

#SCPH06 I Botany

Different organisms may preferentially use different codons to encode the same amino acid in the cases of such amino acids that are encoded by more than one codon this is called

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#Question id: 15237

#SCPH28 | Zoology

Agarose gels are used for the electrophoresis of