Using codon table, identify a 5′ → 3′ sequence of nucleotides in the DNA template strand for an mRNA coding for the polypeptide sequence Phe-Pro-Lys.

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#Question id: 13057

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 382

#SCPH05 I Biotechnology

The molar ratio of sodium acetate to acetic acid in a buffer solution with a pH OF 5.76 is 10:1. Assuming the total buffer concentration is 2.2 X 10^-2 mol/L, how many grams of sodium acetate (m.w. 82) and how many grams of acetic acid (m.w. 60) should be used in preparing a litre of the solution?

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#Question id: 371

#SCPH01 Biochemistry

How many milliliters of a 2.0 M solution of boric acid must be added to 600 mL of a solution of 10 mM sodium borate in order for the pH to be 9.45? (pKa of boric acid = 9.24)

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#Question id: 478

#SCPH05 I Biotechnology

The steps of glycolysis between glyceraldehyde 3-phosphate and 3-phosphoglycerate involve all of the following except:

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#Question id: 19708

#SCPH01 Biochemistry

In complete heart block