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TLS Online TPP Program

#Question id: 4338

Altering patterns of gene expression in prokaryotes would most likely serve an organism's survival by __.

#SCPH06 I Botany

  1. organizing gene expression, so that genes are expressed in a given order

  2. allowing each gene to be expressed an equal number of times

  3. allowing an organism to adjust to changes in environmental conditions

  4. allowing environmental changes to alter a prokaryote's genome

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TLS Online TPP Program

#Question id: 13063

#SCPH06 I Botany

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the statement is worng? Aminopetrin………s
TLS Online TPP Program

#Question id: 13037

#SCPH05 I Biotechnology

Which of the following proteins will be most far away from the positively charged electrode during isoelectric focusing in a buffer with pH of 5?
TLS Online TPP Program

#Question id: 12938

#SCPH06 I Botany

What is the main advantage of using Yeast Artificial Chromosome based vector over other vectors?
1. It has ability to clone very large fragments of DNA.
2. YAC vectors do not contain origin of replication sites 
3. It has insert  range up to 2000 kb.
4. It help in genome mapping and sequencing projects.
TLS Online TPP Program

#Question id: 10288

#SCPH05 I Biotechnology

 If all the ATP are radiolabelled in the cytosol, then predict about radioactivity of each compound.

 a) All enzyme  of glycolysis will be radiolabelled except 2-phophoglycerate and phosphenol pyruvate

 b) 2-phophoglycerate and phosphenol pyruvate are radiolabelled only the next cycle of glycolysis after cytosolic ATP radiolabelling

 c) 1,3 bisphosphoglycerate will be radiolabelled at 1 and 3 both position

 d) 2-phosphoglycerate are not radiolabelled because its enzyme phosphoglycerate mutase active site contain histidine residue exchange phosphate group from 3-phosphoglycerate

 which prediction about radiolabelling is incorrect?
TLS Online TPP Program

#Question id: 4952

#SCPH28 | Zoology

Several scientific laboratories across the globe are involved in research concerning the origin of life on Earth. Which of these questions is currently the most problematic and would have the greatest impact on our understanding if we were able to answer it?