Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the operator to the far end of the operon, past the transacetylase (lacA) gene, which of the following processes would likely occur when the cell is exposed to lactose?

TLS Online TPP Program

#Question id: 13076

#SCPH28 | Zoology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

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#Question id: 13077

#SCPH01 Biochemistry

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13077

#SCPH05 I Biotechnology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13077

#SCPH06 I Botany

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13077

#SCPH28 | Zoology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13078

#SCPH01 Biochemistry

RFLPs have several unique advantages except this one;