TLS Online TPP Program
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TLS Online TPP Program
#Question id: 1563
#SCPH05 I Biotechnology
A nonfunctional CD4 protein on a helper T cell would result in the helper T cell being unable to ________.
TLS Online TPP Program
#Question id: 667
#SCPH05 I Biotechnology
Which represents the backbone of a protein?
Note: R = amino acid side chain
N = nitrogen
Cα = alpha carbon
C = carbonyl carbon
TLS Online TPP Program
#Question id: 3676
#SCPH01 Biochemistry
Which function can be carried out by DNA replication proteins?
TLS Online TPP Program
#Question id: 12946
#SCPH05 I Biotechnology
Choose the incorrect statement for the Multiple Ligation dependent probe amplification.
TLS Online TPP Program
#Question id: 13055
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?