Genetic variation in bacterial populations cannot result from

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#Question id: 6966

#I Life Science/ Life Sciences Group – I-V

During gastrulation in the frog, the very first cells to move into the interior of the embryo through the blastopore come from the surface layer of cells in the marginal zone; they will become:

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#Question id: 11965

#I Life Science/ Life Sciences Group – I-V

Which of the following organs is correctly paired with its function?

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#Question id: 23144

#SCPH06 I Botany

An optical measurement of a protein is taken through microscopy by given that protein a high energy light and it emits a low energy light after absorption. What phenomenon is taking place?

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#Question id: 5047

#I Life Science/ Life Sciences Group – I-V

The process of commitment can be divided into following two stages-

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#Question id: 13054

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?