#Question id: 11980
#SCPH28 | Zoology
#Question id: 14162
#SCPH05 I Biotechnology
A continuous process is set up for treatment of wastewater. Each day, 105 kg cellulose and 103 kg bacteria enter in the feed stream, while 104 kg cellulose and 1.5 x 104 kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x 104 kg d-1. The rate of bacterial growth is 2 x 104 kg d-1 ; the rate of cell death by lysis is 5 x 102 kg d-1. Write balances for cellulose and bacteria in the system.
#Question id: 1730
#SCPH01 Biochemistry
What is the T-cell ligand binding B7 on a professional antigen-presenting cell?
#Question id: 554
#SCPH06 I Botany
The Lineweaver-Burk plot is used to:
#Question id: 13076
#SCPH06 I Botany
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.