Triploids are usually autopolyploids. They arise spontaneously in nature, but they can be constructed by geneticists from the cross of a 4n (tetraploid) and a 2n (diploid). Triploids are characteristically sterile due to

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#Question id: 15616

#SCPH01 Biochemistry

The diagram below shows the F factor and a portion of the E. coli chromosome that has three different insertion sequences (IS) of the same type as is carried on F.

                     

Describe the three different Hfrs (Hfr #1, Hfr #2 and Hfr #3) that can be formed by recombination between the IS on F and each of the IS sequences on the chromosome. Some including the positions of each of the markers (A, B, C, and D) and state which of these markers would be transferred early?

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#Question id: 15616

#SCPH06 I Botany

The diagram below shows the F factor and a portion of the E. coli chromosome that has three different insertion sequences (IS) of the same type as is carried on F.

                     

Describe the three different Hfrs (Hfr #1, Hfr #2 and Hfr #3) that can be formed by recombination between the IS on F and each of the IS sequences on the chromosome. Some including the positions of each of the markers (A, B, C, and D) and state which of these markers would be transferred early?

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#Question id: 15616

#SCPH28 | Zoology

The diagram below shows the F factor and a portion of the E. coli chromosome that has three different insertion sequences (IS) of the same type as is carried on F.

                     

Describe the three different Hfrs (Hfr #1, Hfr #2 and Hfr #3) that can be formed by recombination between the IS on F and each of the IS sequences on the chromosome. Some including the positions of each of the markers (A, B, C, and D) and state which of these markers would be transferred early?

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#Question id: 15617

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 15617

#SCPH06 I Botany

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 15617

#SCPH28 | Zoology

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;