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TLS Online TPP Program
#Question id: 16134
#SCPH01 Biochemistry
You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.
TLS Online TPP Program
#Question id: 19138
#I Life Science/ Life Sciences Group – I-V
Which of the following statement is correct?
TLS Online TPP Program
#Question id: 27189
#SCPH05 I Biotechnology
How long will it take for the initial concentration of A to fall from 0.20 M to 0.075, if the rate constant for a reaction of zero-order in A is 0.035 mol L–1 s–1.
TLS Online TPP Program
#Question id: 7018
#SCPH01 Biochemistry
On the extracellular side, integrins bind to the amino acid sequence, found in several extracellular matrix adhesive proteins. The amino acid sequence is:
TLS Online TPP Program
#Question id: 1760
#SCPH05 I Biotechnology
Effector memory T-cells can be distinguished from the central memory T-cells from which they arise by: