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TLS Online TPP Program
#Question id: 1430
#SCPH28 | Zoology
2-hydroxyglutarate is produced in cancer cells:
TLS Online TPP Program
#Question id: 27864
#Research Methodology
The ____ feature of MS Excel quickly completes a series of data
TLS Online TPP Program
#Question id: 3460
#SCPH06 I Botany
The many eyes hypothesis suggests prey flock because:
TLS Online TPP Program
#Question id: 1048
#SCPH06 I Botany
A virion is composed of
TLS Online TPP Program
#Question id: 13087
#SCPH05 I Biotechnology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?