Several scientific laboratories across the globe are involved in research concerning the origin of life on Earth. Which of these questions is currently the most problematic and would have the greatest impact on our understanding if we were able to answer it?

TLS Online TPP Program

#Question id: 823

#I Life Science/ Life Sciences Group – I-V

chaotropic agents such as guanidinium ion and urea, the efficiency of the chaotropic agent is measured in terms of-

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#Question id: 11076

#I Life Science/ Life Sciences Group – I-V

Which of the following will decrease pulmonary blood flow resistance?

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#Question id: 1555

#SCPH05 I Biotechnology

which type of amino acid in polypeptide position 9 is favoured interaction which MHC class 1?

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#Question id: 15276

#SCPH28 | Zoology

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

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#Question id: 13057

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?