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TLS Online TPP Program
#Question id: 13058
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?
TLS Online TPP Program
#Question id: 591
#SCPH05 I Biotechnology
The Michaelis constant, Km, is equal to the ________.
TLS Online TPP Program
#Question id: 3687
#SCPH05 I Biotechnology
As the two strands of the double helix are separated, the positive supercoiling interferes with the further unwinding of DNA. Which of the following enzyme makes a break in a strand of DNA to release the supercoiling and facilitate the replication to occur?
TLS Online TPP Program
#Question id: 3715
#SCPH28 | Zoology
DNA polymerase III requires a/an ________ for synthesis of DNA to occur.
TLS Online TPP Program
#Question id: 1650
#SCPH05 I Biotechnology
Recombination of V, D, and J Ig gene segments: