Practice Questions

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#Question id: 5214

What is the most probable explanation for the continued presence of pseudogenes in a genome such as our own?

#SCPH06 I Botany

They are genes that had a function at one time, but that have lost their function because they have been translocated to a new location.
They are genes that have accumulated mutations to such a degree that they would code for different functional products if activated.
They are duplicates or near duplicates of functional genes but cannot function because they would provide inappropriate dosage of protein products.
They are genes that are not expressed, even though they have nearly identical sequences to expressed genes.

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#Question id: 3266

#SCPH28 | Zoology

One species mates at the season when daylight is increasing from 13 hours to 13 hours, 15 minutes; another species mates at the season when daylight is increasing from 14 hours to 14 hours, 15 minutes. The isolating mechanism is

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#Question id: 16128

#SCPH01 Biochemistry

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.

Next, you decide to evaluate the promoter for the GLN1 gene. To do this you first fuse the promoter region to the LacZ coding sequence and then place this hybrid gene on an appropriate yeast plasmid. You find that cells carrying the hybrid gene express activity under the same conditions that GS is expressed in wild type cells, meaning that the promoter region you have selected contains all of the necessary cis-acting sequences for normal regulation. The figure below shows the effect of different 50 bp deletions in the promoter region on the amount of ß-galactosidase activity expressed by the reporter gene. how would you expect a gln2– gln3– double mutant to behave?

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#Question id: 13110

#SCPH28 | Zoology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

Match the babies up with their actual parents.

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