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#Question id: 13054
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 16009
#SCPH05 I Biotechnology
BLAST uses a ______ to find matching words, whereas FASTA identifies identical matching words using the ______.
TLS Online TPP Program
#Question id: 5539
#I Life Science/ Life Sciences Group – I-V
When does neurulation occur during human pregnancy?
TLS Online TPP Program
#Question id: 13040
#SCPH01 Biochemistry
Which of the following is incorrect about Oligonucleotide Design in A microarray?
TLS Online TPP Program
#Question id: 23249
#SCPH28 | Zoology
Which enzyme converts the PPi to ATP in Pyrosequencing?