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TLS Online TPP Program

#Question id: 13023

Which of the following enzymes are required for end labelling?

#SCPH06 I Botany
  1. Alkaline phosphatase and Polynucleotide kinase
  2. Alkaline phosphatase and DNA polymerase
  3. Polynucleotide kinase and DNA polymerase
  4. Polynucleotide kinase and DNA ligase
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TLS Online TPP Program

#Question id: 1637

#I Life Science/ Life Sciences Group – I-V

Protective antibodies against infectious agents are often:
TLS Online TPP Program

#Question id: 13058

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?
TLS Online TPP Program

#Question id: 15561

#SCPH06 I Botany

Which mechanism is helpful in secondary metabolite production
1. ppGpp secretion
2. Autoinducer
3. Nutrient limitation
4. Senescence genes
Which combination is incorrect  from the following.
TLS Online TPP Program

#Question id: 10524

#I Life Science/ Life Sciences Group – I-V

Members of the Poaceae family, which includes all of the cereal crops, use benzoxazinoids, these alkaloids shows;

a) Alkaloids are derived from the tryptophan as constitutively produced defensive secondary metabolites

b) These benzoxazinoids such as DIBOA and its derivative DIMBOA are usually stored in the peroxisome

c) Upon damage, the inactive glycosides are hydrolyzed, generating glucosinolate, which are toxic not only to insect herbivores but also against pathogens

d) DIBOA and DIMBOA perform insecticidal and pesticidal activities

e) It can be inactivated by hydroxylation and N-glycosylation reactions

Which of the following combination is correct about benzoxazinoids alkaloids?
TLS Online TPP Program

#Question id: 468

#SCPH05 I Biotechnology

Glycolysis is the name given to a metabolic pathway occurring in many different cell types.  It consists of 11 enzymatic steps that convert glucose to lactic acid.  Glycolysis is an example of