Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

TLS Online TPP Program

#Question id: 15730

#SCPH01 Biochemistry

Haploid plants may be obtained from culturing

1) Anther culture

2) Pollen culture

3) Stem culture

4) Ovary culture

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#Question id: 5064

#SCPH01 Biochemistry

Fibroblast growth factors, epidermal growth factors, platelet-derived growth factors, and stem cell factor are all paracrine factors that bind to

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#Question id: 4941

#SCPH06 I Botany

Which of the factors below weaken the hypothesis of abiotic synthesis of organic monomers in early Earthʹs atmosphere?

1.  the relatively short time between intense meteor bombardment and appearance of the first life forms

2.  the lack of experimental evidence that organic monomers can form by abiotic synthesis

3.  Uncertainty about which gases comprised early Earthʹs atmosphere

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#Question id: 18713

#SCPH01 Biochemistry

How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?

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#Question id: 3873

#SCPH01 Biochemistry

The excision (splicing) of many group I introns requires, in addition to the primary transcript RNA: