Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 10685

#SCPH06 I Botany

figure show population growth in two species A ana B . Interaction between

 

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#Question id: 2085

#SCPH05 I Biotechnology

Cholesterol mixes with phospholipids in a biomembrane because cholesterol molecules are

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#Question id: 3663

#SCPH05 I Biotechnology

_______________ is a specific RNase which cuts RNA–DNA hybrids; involved in replication of ColE1-like plasmids.

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#Question id: 9212

#SCPH28 | Zoology

Burning fossil fuels releases oxides of sulfur and nitrogen. These air pollutants can be responsible for ________.

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#Question id: 11408

#SCPH06 I Botany

According to IUCN red list criteria , a species would be classified on the basis of following criteria are a- >50 decline in population during last 10 year b-occur in area less than 20,000 sq km c- total adult population less than 2500 Above observation which of the following class of IUCN