Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 8937

#SCPH28 | Zoology

During metamorphosis, echinoderms undergo a transformation from motile larvae to a sedentary (or sometimes sessile) existence as adults. What differentiates echinoderm adults, but not their larvae? Adults should

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#Question id: 4584

#SCPH28 | Zoology

When Cuvier considered the fossils found in the vicinity of Paris, he concluded that the extinction of species ________.

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#Question id: 6947

#SCPH06 I Botany

What is the correct dorso-ventral arrangement seen in a cross-section of Drosophila embryo?

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#Question id: 5596

#SCPH01 Biochemistry

The functional maturation of sperm after they have been deposited in the female reproductive tract, known as

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#Question id: 2961

#I Life Science/ Life Sciences Group – I-V

What is the best evidence that anaphase chromosomes are not pulled to the poles like fish on a line?