Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

#Question id: 10158

#I Life Science/ Life Sciences Group – I-V

Following are some statements regarding CO2 assmilation;

a.) The reduction process-Hydroxypyruvate convert into Glycerate in Peroxisomes in Glycolate pathway

b.) In Calvin-Benson cycle action of Transketolase produces erythrose 4-phosphate and xylulose 5-phosphate during regeneration step

c.) In C4 cycle phosphoenolpyruvate carboxylase (PEPCase), catalyzes the initial carboxylation in mesophyll cells close to the external atmosphere

d.) The stomata of CAM plants open at night

Which of the following above statements combination TRUE?

#Question id: 10159

#I Life Science/ Life Sciences Group – I-V

The ambient temperature exerts an important influence over Ω and the concentrations of CO2 and O2 around the active site of rubisco. Warmer environments have the effect of;  Find the incorrect one?

#Question id: 10160

#I Life Science/ Life Sciences Group – I-V

Which of the following statements about the C4 cycle is true ?

a.) C4 plants uses the enzyme phosphoenolpyruvate carboxylase (PEPCase), rather than rubisco

b.) Unlike rubisco, O2 does not compete with HCO3 − in the carboxylation catalyzed by PEPCase

c.)  initial carboxylation catalyzes in bundle sheath cells, four-carbon acids formed and flow across the diffusion barrier to the mesophyll cells

d.) during decarboxylation, releasing CO2 that is refixed by rubisco via the Calvin–Benson cycle

#Question id: 10164

#I Life Science/ Life Sciences Group – I-V

C4 photosynthetic carbon cycle is also known as the

#Question id: 10165

#I Life Science/ Life Sciences Group – I-V

In the C4 photosynthetic carbon cycle involves uptake of CO2 in the form of HCO₃^-, the acceptor of the HCO₃^- is

#Question id: 10167

#I Life Science/ Life Sciences Group – I-V

Mechanisms of C4 acid transported mesophyll → bundle sheath for decarboxylation, which of the following CORRECT combination of decarboxylation process?

a.) NADP–malic enzyme (NADP–ME)→ transport Malate from mesophyll to bundle sheath

b.) NAD–malic enzyme (NAD–ME)→ transport Aspartate from mesophyll to bundle sheath

c.) PEP carboxykinase (PEPCK)→ transport Aspartate from mesophyll to bundle sheath

d.) PEP carboxylase (PEPCase)→ transport Malate from mesophyll to bundle sheath