Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

#Question id: 10798

#I Life Science/ Life Sciences Group – I-V

Two other major groups of flavonoids found in flowers and leaves of all green plants. The function of these two flavonoids in plants are____

a) protect plants against being eaten by herbivores and against being infected by microbial pathogens

b) Protect cells from excessive UV-B radiation (280–320 nm) because they accumulate in the epidermal layers of leaves and stems and absorb light strongly in the UV-B region       

c) flavones and flavonols secreted into the soil by legume roots mediate the interaction of legumes and nitrogen-fixing symbionts

d) They are are coloured terpenoid compounds that also serve as accessory pigments in photosynthesis    

Which of the following combination from the above statements is correct?

#Question id: 788

#SCPH06 I Botany

Which of the following is true about Z-DNA helix?

#Question id: 549

#SCPH05 I Biotechnology

Which one of the following statements is true of enzyme catalysts?

#Question id: 2613

#SCPH01 Biochemistry

If increasing the number of trp codon,

#Question id: 8898

#SCPH28 | Zoology