Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

TLS Online TPP Program

#Question id: 8920

#SCPH06 I Botany

Almost all of the major animal body plans seen today appeared in the fossil record over 500 million years ago at the beginning of the

TLS Online TPP Program

#Question id: 8920

#SCPH28 | Zoology

Almost all of the major animal body plans seen today appeared in the fossil record over 500 million years ago at the beginning of the

TLS Online TPP Program

#Question id: 8921

#I Life Science/ Life Sciences Group – I-V

Evidence of which structure or characteristic would be most surprising to find among fossils of the Ediacaran fauna?

TLS Online TPP Program

#Question id: 8921

#SCPH05 I Biotechnology

Evidence of which structure or characteristic would be most surprising to find among fossils of the Ediacaran fauna?

TLS Online TPP Program

#Question id: 8921

#SCPH06 I Botany

Evidence of which structure or characteristic would be most surprising to find among fossils of the Ediacaran fauna?

TLS Online TPP Program

#Question id: 8921

#SCPH28 | Zoology

Evidence of which structure or characteristic would be most surprising to find among fossils of the Ediacaran fauna?