Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

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#Question id: 10352

#SCPH06 I Botany

which of the following amino acid that participates in the malate–aspartate shuttle to transfer reducing equivalents from the mitochondrion and chloroplast into the cytosol?

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#Question id: 9172

#SCPH06 I Botany

Two plant species live in the same biome but on different continents. Although the two species are not at all closely related, they may appear quite similar as a result of ________.

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#Question id: 13056

#SCPH28 | Zoology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

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#Question id: 22956

#I Life Science/ Life Sciences Group – I-V

Individual and overlapping expression of homeotic genes in adjacent whorl of a flower determine the pattern of floral organ development. In an Arabidopsis mutant, floral organs are distributed as follows

WHORL 1 Sepal

WHORL 2 Petal

WHORL 3 Petal

WHORL 4 Sepal

Loss of function mutation in which one of the following genes would have caused the above pattern of floral organs development 

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#Question id: 3614

#SCPH12 I Genetics

A gene showing codominance–