Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

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#Question id: 2612

#SCPH01 Biochemistry

Which statement is correct regarding regulation of trp operon;

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#Question id: 4724

#SCPH28 | Zoology

Which of the following are true about crossing over ?

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#Question id: 12990

#SCPH06 I Botany

Choose the incorrect statement related to ionic interactions

a. Ionic interactions takes place between positively and negatively charged amino acids side chain.

b. Ionic interactions takes place between positively and positively charged amino acids side chain.

c. ionic interaction has almost 20 kj/mol of bond energy.

d. ionic interaction is weak interaction that have lowest 20kj/mol of bond energy.

e. strength of ionic bond within protein chain calculated by

 

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#Question id: 3643

#SCPH05 I Biotechnology

One DNA strand, the______________, is made continuously in a 5→3 direction from the origin.

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#Question id: 127

#SCPH05 I Biotechnology

Which two organelles are responsible for producing phospholipids and sphingolipids?