To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 2298

#SCPH01 Biochemistry

The endoplasmic reticulum and ribosomes are both involved in   

#Question id: 1556

#SCPH06 I Botany

Which one of these is not a property of Antigen?

#Question id: 829

#I Life Science/ Life Sciences Group – I-V

Which one is the incorrect conclusion of Anfinsen’s eperiments?

#Question id: 12944

#SCPH28 | Zoology

#Question id: 10535

#SCPH05 I Biotechnology

The insect ingests plant tissue containing the fatty acids, an enzyme in the gut conjugates the plant-derived fatty acid to an insect-derived amino acid to form an elicitor, When plants recognize elicitors from insect saliva induces some typical responses such as

a) Increasing Ca²⁺ levels in the cytosol and then activate an array of target proteins, such as calmodulin and other Ca²⁺-dependent protein kinases

b) In Arabidopsis, a calmodulin-binding transcriptional regulator called IQD1 was identified as an important mediator of defense responses against insect herbivory

c) overexpression of IQD1 in Arabidopsis activate the herbivore activity

d) IQD1 binds calmodulin, a major Ca2+-binding protein, in a Ca2+-dependent manner and subsequently inhibits the gene involved in glucosinolate biosynthesis

Which of the following combination from above statements is correct?