To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

#Question id: 3055

#SCPH06 I Botany

300 CFU will form on petriplate if 0.1ml sample plating from 10^6 diluted sample. Original sample contain bacteria per ml is

#Question id: 3056

#SCPH05 I Biotechnology

Ten bacteria were inoculated into a rich medium. If at the end of ten hours the total number of cells is 10^4, then the number of elapsed generations and the generation time respectively is

#Question id: 3056

#SCPH06 I Botany

Ten bacteria were inoculated into a rich medium. If at the end of ten hours the total number of cells is 10^4, then the number of elapsed generations and the generation time respectively is

#Question id: 3057

#SCPH06 I Botany

Mean generation time of bacteria is 20 min. 6 hours of incubation, the bacterial population increases from 10^3 cells to

#Question id: 3058

#SCPH05 I Biotechnology

A urine sample with more than 100,000 organisms is considered indicative of infection. A urine sample containing 5,000 bacteria, with a generation time of 30 minutes, sits for 3 hours before finally being assayed. How many bacteria will then be present within the sample

#Question id: 3058

#SCPH06 I Botany

A urine sample with more than 100,000 organisms is considered indicative of infection. A urine sample containing 5,000 bacteria, with a generation time of 30 minutes, sits for 3 hours before finally being assayed. How many bacteria will then be present within the sample