To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 1092

#SCPH06 I Botany

Which of the following statements is true of steroid receptors?

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#Question id: 1413

#SCPH05 I Biotechnology

Which of the following is a feature that defines a cell dying in response to tissue damage (necrosis)?

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#Question id: 18640

#I Life Science/ Life Sciences Group – I-V

Bootstrapping technique in molecular phylogeny analysis is used to derive additional datasets by

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#Question id: 16485

#SCPH01 Biochemistry

A modification of the original grid method is widely used to study the 

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#Question id: 20713

#SCPH28 | Zoology

Under anaerobic conditions and in the presence of what microbial electron transfer and respiration can proceed if suitable exogenous electron acceptors are available