With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

 

 Why is it that having more repeats leads to a band that is higher in the gel?

#Question id: 14300

#SCPH05 I Biotechnology

A 15-m ³ chemostat is operated with dilution rate 0.1 h^-1. A continuous steriliser with steam injection and flash cooling delivers sterilized medium to the fermenter. Medium in the holding section of the steriliser is maintained at 130⁰C The concentration of contaminants in the raw medium is 10⁵ ml^-l; an acceptable contamination risk is one organism every 3 months. The Arrhenius constant and activation energy for thermal death are estimated to be 7.5 × 10³⁹ h^-1 and 288.5 kJ gmol^{- 1}, respectively. The steriliser pipe inner diameter is 12 cm. At 130⁰C the liquid density is 1000 kg m^{- 3} and the viscosity is 4 kg m^{- 1} h^{- 1}. Assuming perfect plug flow, determine the length of the holding section.   __________________

#Question id: 14302

#SCPH05 I Biotechnology

Pseudomonas sp. has a mass doubling time of 2.4 h when grown on acetate. The saturation constant using this substrate is 1.3 g/l (which is unusually high), and cell yield on acetate is 0.46 g cell/g acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate, find the Substrate concentration when the dilution rate is 0.8Dmax?

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#Question id: 14303

#SCPH05 I Biotechnology

Pseudomonas sp. has a mass doubling time of 2.4 h when grown on acetate. The saturation constant using this substrate is 1.3 g/l (which is unusually high), and cell yield on acetate is 0.46 g cell/g acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate, find the Maximum dilution rate?

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#Question id: 14304

#SCPH05 I Biotechnology

Pseudomonas sp. has a mass doubling time of 2.4 h when grown on acetate. The saturation constant using this substrate is 1.3 g/l (which is unusually high), and cell yield on acetate is 0.46 g cell/g acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate, find the Cell productivity at 0.8 Dmax?

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#Question id: 14305

#SCPH05 I Biotechnology

Ethanol is to be used as a substrate for single-cell protein production in a chemostat. The available equipment can achieve an oxygen transfer rate of 10 g O₂/l of liquid per hour. Assume the kinetics of cell growth on ethanol is of the Monod type, with µm = 0.5 h^–1, K_s = 30 mg/l, YX/S = 0.5 cells/g ethanol, and YO2/S = 2 g O2/g EtOH. We wish to operate the chemostat with an ethanol concentration in the feed of 22 g/L. We also wish to maximize the biomass productivity and minimize the loss of unused ethanol in the effluent. Determine the required dilution rate