Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 1395

#SCPH28 | Zoology

Which of the following mutations would promote malignancy in cells whose proliferation is inhibited by TGFβ?

a. overexpression of Smad2               

b. loss of Smad 4

c. overexpression of TGFβ receptor I

d. loss of TGFβ receptor II

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#Question id: 18720

#SCPH01 Biochemistry

The derivatives of M13 vector are exact counterparts of _________

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#Question id: 22945

#SCPH06 I Botany

Suppressor of overexpression of constans1 (SOC1) that is expressed in Leaves and apex, belongs to which, transcription factor family

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#Question id: 122

#SCPH05 I Biotechnology

An unknown lipid is treated with a mixture of phospholipases A1, A2, C and D. Since no glycerol is formed after this treatment, the lipid is most likely

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#Question id: 5547

#SCPH01 Biochemistry

 Cells that rove from somites form the