TLS Online TPP Program

#Question id: 15653


You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation on the basis of genetic properties,  make a proposal for the type of regulatory functions affected by the MelA– mutation?

#SCPH06 I Botany
  1. MelA is likely a constitutive regulator of Mel1 expression.
  2. MelA is likely a positive regulator of Mel1 expression.
  3. MelA is likely a negative regulator of Mel1 expression.
  4. MelA is likely a repressive regulator of Mel1 expression.