You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. Next you isolate a mutation, designated MelB–, which gives uninducible melibiase activity. Mapping experiments show that MelB– is linked to Mel1. Using an F' factor that carries the chromosomal region surrounding Mel1, you perform the following genetic tests:

 

Describe the proposal for the type of regulatory functions affected by the MelB– mutation.

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#Question id: 18664

#SCPH06 I Botany

2D electrophoresis is the combination of 

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#Question id: 18665

#SCPH01 Biochemistry

In a road accident a forensic scientist collected traces of blood or other biological fluids and he wants to compared samples according to the composition of certain isoenzymes, for this which technique will efficiently work

TLS Online TPP Program

#Question id: 18665

#SCPH06 I Botany

In a road accident a forensic scientist collected traces of blood or other biological fluids and he wants to compared samples according to the composition of certain isoenzymes, for this which technique will efficiently work

TLS Online TPP Program

#Question id: 18666

#SCPH01 Biochemistry

The above figure shows where sample applied was 100 µg of total protein extracted from a normal dog heart ventricle. The first dimension was conducted using a pH 4–7 isoelectric focussing gel. The second dimension was a 12% SDS–PAGE vertical slab gel. The pattern was visualised by silver staining. Which of the following techniques is applied

TLS Online TPP Program

#Question id: 18666

#SCPH06 I Botany

The above figure shows where sample applied was 100 µg of total protein extracted from a normal dog heart ventricle. The first dimension was conducted using a pH 4–7 isoelectric focussing gel. The second dimension was a 12% SDS–PAGE vertical slab gel. The pattern was visualised by silver staining. Which of the following techniques is applied

TLS Online TPP Program

#Question id: 18667

#SCPH01 Biochemistry

Which of the following is highly sophisticated analytical method for analysing protein mixtures