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TLS Online TPP Program

#Question id: 16129

5 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.
Describe the cis-acting elements in the GLN1 promoter that are evident from these experiments, giving both their position and as much of their function as you can deduce.

#SCPH06 I Botany
  1. Deletion1 : GLN2 function, Deletions 3, 4 : GLN3 function, contain TATA, Deletion 6: sequence contain UAS Sequence -involved in activation.
  2. Deletion1 : GLN3 function, Deletions 3, 4 : GLN2 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
  3. Deletion1 : GLN2 function, Deletions 3, 4 : GLN3 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
  4. Deletion1, 3 and 4 : GLN3 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
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TLS Online TPP Program

#Question id: 5044

#SCPH28 | Zoology

The cell might not look different from its nearest or most distant neighbors in the embryo and show no visible signs of differentiation, but its developmental fate has become restricted, the process known as-
TLS Online TPP Program

#Question id: 20987

#SCPH28 | Zoology

Toluene degradation could be optimized and linked to the reduction of 
1. Nitrate 
2. Nitrous oxide
3. Nitrite
Which of the below combinations are correct?
TLS Online TPP Program

#Question id: 1296

#SCPH28 | Zoology

People with a defect in the synthesis of the integrin β2 subunit exhibit reduced:
TLS Online TPP Program

#Question id: 19917

#SCPH01 Biochemistry

Antigen-Antibody interactions are__
TLS Online TPP Program

#Question id: 15622

#SCPH01 Biochemistry

9 In your study of a new bacterial species you have identified a transducing phage that you call Px. In addition you have worked out methods to make random transposon insertions into the bacterial genome. You have generated two different transposon insertion collections one with 105 random Tn5 (Kanr) insertions and the other with 105 random Tn10 (Tetr) insertions. You grow Px phage on the mixed collection of Tn5 insertions and use the resulting phage lysate to infect the mixed collection of Tn10 insertions. You select 10,000 Kanr  transductants and find that 80 of them are Tets. Use this information to estimate the total size of the bacterial genome assuming that both Tn5 and Tn10 insert randomly and that the average size of a fragment recombined into the recipient genome during Px transduction is 55 kbp. (Tn5 is about 5 kbp and Tn10 is 10 kbp.)