You are running a human assisted reproduction clinic and providing state-of-the-art genetic diagnostic services. A married couple who already had a child with cystic fibrosis approach you because they wish to have another child, but only if they can be assured that the child will not have cystic fibrosis. You genotype the woman and discover that she is a heterozygote for Del508, the most common mutation causing cystic fibrosis. You suggest that the couple consider first polar body testing, in which several unfertilized oocytes (each with its first polar body) are retrieved from the woman, the first polar bodies are removed, and PCR tests are conducted on DNA from each of the first polar bodies. The couple agrees, and you obtain the following results:

 

the observation that the polar bodies for oocytes #1 and #5 test positive for both Del508 and the wild type sequence, because

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#Question id: 14284

#SCPH05 I Biotechnology

The specific productivity (qp) of an enzyme production Is fitted linearly with specific growth rate p of a fungal organism according to the equation The estimated values of constants alpha and beta are 0.0006 and 25 respectively. The enzyme production kinetics is 

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#Question id: 14285

#SCPH05 I Biotechnology

Match the transport process with appropriate time constants given below 

Transport process                       time constants

1. Oxygen transfer                     p. V/Q

2. Heat transfer                      q. 4V(1.5ND3)

3. Flow                                     r. VpCp/UA

4. Mixing                              s. 1/KLa 

TLS Online TPP Program

#Question id: 14285

#SCPH05 I Biotechnology

Match the transport process with appropriate time constants given below 

Transport process                       time constants

1. Oxygen transfer                     p. V/Q

2. Heat transfer                      q. 4V(1.5ND3)

3. Flow                                     r. VpCp/UA

4. Mixing                              s. 1/KLa 

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#Question id: 14286

#SCPH05 I Biotechnology

 When a liquid of density 1200kg/m3 and viscosity 0.01 kg m^-1s^-1 flows through a 1 cm ID pipe at 0.2m/s, the flow is referred as a 

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#Question id: 14286

#SCPH05 I Biotechnology

 When a liquid of density 1200kg/m3 and viscosity 0.01 kg m^-1s^-1 flows through a 1 cm ID pipe at 0.2m/s, the flow is referred as a 

TLS Online TPP Program

#Question id: 14287

#SCPH05 I Biotechnology

A chemostat is run with a feed rate of llitre/h when the volume of the reactor is also 1 litre. At steady state the doubling time of the cells in the chemostat is.....   h.