You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   

 

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

 

Would you expect the DNA sequence of PCR product obtained at STS5 using mouse genomic DNA as template to more closely resemble that obtained using BAC B, BAC D, or BAC F? Briefly explain your answer.

#Question id: 6966

#SCPH06 I Botany

During gastrulation in the frog, the very first cells to move into the interior of the embryo through the blastopore come from the surface layer of cells in the marginal zone; they will become:

#Question id: 14297

#SCPH05 I Biotechnology

A two-stage chemostat system is used for production of secondary metabolite. The volume of each reactor is 0.5 m³; the flow rate of feed is 50 l h^{- 1}. Mycelial growth occurs in the first reactor; the second reactor is used for product synthesis. The concentration of substrate in the feed is 10 gl^-1. Kinetic and yield parameters for the organism are:

Assume that product synthesis is negligible in the first reactor and growth is negligible in the second reactor. Determine substrate concentrations entering the second reactor.

#Question id: 9196

#SCPH06 I Botany

If all individuals in the last remaining population of a particular frog species were all highly elated, which type of diversity would be of greatest concern when planning to prevent the species from going extinct?

#Question id: 9233

#SCPH06 I Botany

What is the biggest problem with selecting a site for a reserve?

#Question id: 2648

#SCPH01 Biochemistry

In which microorganism will you find attenuation by alternate loop formation due to ribosomal stalling?