Practice Questions

TLS Online TPP Program

#Question id: 16137

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

How would you use the sequence information presented in to design two new STSs (with new PCR primer pairs) to replace STS5? (Call the new ones STS51 and STS52. STS51 should be present (+) in BAC B, and STS52 should be present (+) in BAC F.)

#SCPH06 I Botany

Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS52 not STS51.
Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify both simultaneously STS51 and STS52.
Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS51 not STS52.
Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify either STS51 or STS52.