TLS Online TPP Program

#Question id: 18615


PCR efficiency is influenced by several factors, the efficiency declines due to
a) efficiency of amplification declines with an increase in the length of the target sequence.
b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.
c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.
d)  primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.

#SCPH06 I Botany
  1. only a
  2. only c
  3. a and b only
  4. a, b, c, and d