TLS Online TPP Program

#Question id: 18645


SDS PAGE separate molecules by size because the presence of SDS (sodium dodecyl sulphate) denatures the protein removing 2˚, 3˚ and 4˚ structures (they assume a linear chain) and the SDS coats the molecules giving them a uniform charge/mass ratio. In an experiment (SDS-PAGE) with a discontinuous buffer system following band pattern is obtained 

   
Choose the correct statement regarding this band pattern

#SCPH06 I Botany
  1. Lysozyme shows least mobility
  2. Unknown protein shows highest mobility
  3. myosin has highest molecular mass
  4. myosin has lowest molecular mass
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TLS Online TPP Program

#Question id: 18075

#SCPH06 I Botany

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 18081

#SCPH01 Biochemistry

The interaction of selection and inbreeding in determining the incidence of autosomal recessive diseases. Consider a gene in which recessive mutations occur at a rate of 10-5. Assume a selective disadvantage S of 0.4 in homozygotes for the recessive allele. What is the frequency of the recessive allele (q). And calculate the incidence of the disease. Assume random mating.

TLS Online TPP Program

#Question id: 18081

#SCPH12 I Genetics

The interaction of selection and inbreeding in determining the incidence of autosomal recessive diseases. Consider a gene in which recessive mutations occur at a rate of 10-5. Assume a selective disadvantage S of 0.4 in homozygotes for the recessive allele. What is the frequency of the recessive allele (q). And calculate the incidence of the disease. Assume random mating.

TLS Online TPP Program

#Question id: 18084

#SCPH01 Biochemistry

PSORT is a computational tool which is used for

TLS Online TPP Program

#Question id: 18084

#I Life Science/ Life Sciences Group – I-V

PSORT is a computational tool which is used for

TLS Online TPP Program

#Question id: 18084

#SCPH05 I Biotechnology

PSORT is a computational tool which is used for