In Maxam gilbert method guanine specific cleavage is done by using

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#Question id: 12714

#SCPH06 I Botany

Physiological and biochemical perturbations in plants caused by fluctuations in the abiotic environment, given column I with environmental factor and with its effects in the form of primary effects  in column II and secondary effects  in column III

COLUMN I        (environmental factor )	COLUMN II            (primary effects)	COLUMN III         (secondary effects)
A) Water deficit	i) Hypoxia,  Anoxia	a) Membrane dysfunction
B) High temperature	ii) ROS production	b) Reduced cellular and metabolic activities, Leaf abscission, Ion cytotoxicity and Cavitation
C) Trace element toxicity	iii) Photo inhibition	c) Reduced respiration,     Fermentative metabolism,       Inadequate ATP production ,  ROS production and  Stomatal closure
D) Flooding and soil compaction	iv) Membrane destabilization	d) Inhibition of PSII repair
E) Chilling	v) Cell dehydration and Hydraulic resistance	e) Disruption of metabolism
F) High light intensity		f) Photosynthetic and respiratory inhibition, ROS production

Find out the correct combination of given above column;

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#Question id: 444

#SCPH06 I Botany

Which of the following is not a factor contributing to the large free energy of hydrolysis of ATP?

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#Question id: 1271

#SCPH28 | Zoology

Common sugars found in proteoglycans include all of the following except:

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#Question id: 3003

#I Life Science/ Life Sciences Group – I-V

Which statement best characterizes the behavior of a bacterial culture during EXPONENTIAL GROWTH?

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#Question id: 13088

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;