A chemical is said to be fluorescent if it absorbs light at one wavelength (excitation) and emits light (fluoresces) at a specific 

#Question id: 3534

#SCPH28 | Zoology

Start with a collection of mutants obtained using selections or screens and then characterize their wild functions at the molecular level called ----A------, alternatively, start with a set of specific DNA sequences, mutate them, and observe the phenotypes called --B-----.

#Question id: 10284

#SCPH05 I Biotechnology

Allosteric regulation of enzyme multisubunit or multiactive site shows cooperativity with the sigmoidal graph between substerate conc. v/s Activity,

a) Cell contain NADH, FADH2 and ATP, that inhibits the allosteric enzyme activity by lowering the affinity with its substate, graph shifts towards B from control

 b) A- shows low ATP, the Km for fructose 6-phosphate is relatively low, the affinity of the enzyme with its substate is relatively high

 c) Haemoglobin, Phosphofructokinase, Phosphate dehydrogenase complex, α-ketoglutarate dehydrogenase complex, these all enzymes shows characteristics of sigmoidal graph

 d) When ATP is high, Km for fructose 6-phosphate is greatly increased- this activity shown by B in the graph

 e) fructose 2,6-bisphosphate binds to its allosteric site on PFK-1, it increases the enzyme’s affinity for its substrate fructose 6-phosphate and reduces its affinity for the allosteric inhibitors ATP and citrate- shown in graph by A

 Which of the following  combination of the enzyme activity with its substate correctly shown;

#Question id: 15622

#SCPH28 | Zoology

#Question id: 10250

#SCPH05 I Biotechnology

Glycolysis involves a series of reactions catalyzed by enzymes located in,

#Question id: 23254

#SCPH06 I Botany