What proportional of one round and two wrinkle seed if random selected 3 seed from various seed produced from monohybrid test cross?

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#Question id: 13089

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

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#Question id: 6946

#SCPH28 | Zoology

The adult body plan of the fly is based on reiterating structures called segments; segment precursors (parasegments) are first positioned at the cell-by-cell level by which group of genes?

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#Question id: 1294

#SCPH06 I Botany

Dystroglycan is a large glycoprotein that binds to dystrophin in muscle cells.  It is also present in other cells and can also bind to:

a.  laminin.

b.  the virus that causes Lassa fever.

c.  the bacterium that causes leprosy.

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#Question id: 313

#I Life Science/ Life Sciences Group – I-V

What is the difference between a particle being hydrated versus being solvated?

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#Question id: 11914

#SCPH06 I Botany

Alder trees, which harbour nitrogen-fixing bacteria in their roots, provide nitrogen to soils developing on sandbars rivers, this is an example of,