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TLS Online TPP Program
#Question id: 13062
#SCPH06 I Botany
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?
TLS Online TPP Program
#Question id: 15238
#SCPH28 | Zoology
Electrophoretic resolution can be mainly changed by adjusting the
a) current
b) pH of buffer system
c) luminous intensity
d) Gel concentration
TLS Online TPP Program
#Question id: 18680
#SCPH01 Biochemistry
DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by
TLS Online TPP Program
#Question id: 15486
#SCPH28 | Zoology
Why glutamic acid accumulation in cell is not a good idea for Corynebacterium
TLS Online TPP Program
#Question id: 5040
#SCPH06 I Botany
The establishment of the anterior-posterior or dorsal-ventral body axes is called: