Practice Questions

TLS Online TPP Program

#Question id: 3689

DNA replication is bidirectional and anti-parallel. Which of the statement is FALSE regarding the DNA replication?

#SCPH28 | Zoology

The DNA synthesis i.e., addition of nucleotide occurs from 5'-3' position
The DNA synthesis is semi-continuous with continuous leading strand and discontinuous lagging strand.
The synthesis of leading and lagging strands occurs simultaneously
None of the Above

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TLS Online TPP Program

#Question id: 3522

#SCPH01 Biochemistry

A haplo-diplod insect, Male are haploid but female are diploid, A female who is heterozygous for recessive red eye color mutation mate with red eye male. What will be percent of wild phenotype color eye among their female?

TLS Online TPP Program

#Question id: 3011

#SCPH05 I Biotechnology

Bacteria that may form endospores include

TLS Online TPP Program

#Question id: 13093

#SCPH01 Biochemistry

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?