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#Question id: 16128
#SCPH06 I Botany
You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.
Next, you decide to evaluate the promoter for the GLN1 gene. To do this you first fuse the promoter region to the LacZ coding sequence and then place this hybrid gene on an appropriate yeast plasmid. You find that cells carrying the hybrid gene express activity under the same conditions that GS is expressed in wild type cells, meaning that the promoter region you have selected contains all of the necessary cis-acting sequences for normal regulation. The figure below shows the effect of different 50 bp deletions in the promoter region on the amount of ß-galactosidase activity expressed by the reporter gene. how would you expect a gln2– gln3– double mutant to behave?
TLS Online TPP Program
#Question id: 840
#SCPH01 Biochemistry
Which of the following type of chaperon that are involved in functions to unfold proteins in preparation for their transport through membranes and to subsequently refold them after transport?
TLS Online TPP Program
#Question id: 5751
#SCPH28 | Zoology
A biochemical mutant that must be supplied with a particular nutrient for growth would be described as a(n)
TLS Online TPP Program
#Question id: 19691
#SCPH01 Biochemistry
The QT interval of an electrocardiogram (ECG) includes the
TLS Online TPP Program
#Question id: 13078
#SCPH01 Biochemistry
RFLPs have several unique advantages except this one;