TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 2625
#SCPH01 Biochemistry
positive regulation is applied when
TLS Online TPP Program
#Question id: 15657
#SCPH01 Biochemistry
You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.
You next construct a MelA– MelB– double mutant, which gives the following behavior:
Which of your two models is consistent with this new data?
a) the MelA- MelB- double mutant is identical to the MelB- mutant
b) MelA- is epistatic to MelB-
c) MelB- is epistatic to MelA-
d) the MelA- MelB- double mutant is identical to the MelA- mutant
Which of the following is the correct prediction about the double mutants?
TLS Online TPP Program
#Question id: 8692
#SCPH05 I Biotechnology
Which of the following statements is accurate with regard to a phylogeny, as represented by a phylogenetic tree?
TLS Online TPP Program
#Question id: 13092
#SCPH05 I Biotechnology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?
TLS Online TPP Program
#Question id: 17080
#SCPH01 Biochemistry
Hypoxanthine and thymidine are required for running which pathway