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TLS Online TPP Program
#Question id: 19543
#SCPH05 I Biotechnology
Which sulfate has the highest solubility in water?
TLS Online TPP Program
#Question id: 3876
#SCPH28 | Zoology
Which one of the following is not true of the mRNA for ovalbumin?
TLS Online TPP Program
#Question id: 3857
#SCPH01 Biochemistry
The CAAT box is usually located near the position:
TLS Online TPP Program
#Question id: 15882
#SCPH05 I Biotechnology
Selection of culture media depends on___
TLS Online TPP Program
#Question id: 13087
#SCPH06 I Botany
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?