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#Question id: 13057
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 8887
#SCPH06 I Botany
Generally, members of which flatworm class(es) are nonparasitic?
TLS Online TPP Program
#Question id: 5042
#SCPH01 Biochemistry
Which one of the following set of Yamanaka factors can be used to regained the pluripotency of somatic cells in mammals?
TLS Online TPP Program
#Question id: 5578
#SCPH28 | Zoology
The phase change of an apical meristem from the juvenile to the mature vegetative phase is often revealed by ________.
TLS Online TPP Program
#Question id: 18625
#SCPH06 I Botany
m.RNA variation can be analyzed by using