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TLS Online TPP Program
#Question id: 13056
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is true for traditional and real time PCR?
TLS Online TPP Program
#Question id: 14127
#SCPH05 I Biotechnology
The most basic sequence alignment method is –
TLS Online TPP Program
#Question id: 4180
#SCPH28 | Zoology
The anticodon of tRNAala can bind to three different codons for alanine (GCU, GCC and GCA) because
TLS Online TPP Program
#Question id: 388
#SCPH05 I Biotechnology
Calculate the pH of a solution prepared by mixing 2.0 mL of a strong acid solution of pH=3.0 and 3.0 mL of a strong base of pH=10.0.
TLS Online TPP Program
#Question id: 2102
#I Life Science/ Life Sciences Group – I-V
In which of the following environments would there be the greatest need for osmoregulation?