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#Question id: 556
#I Life Science/ Life Sciences Group – I-V
Which of the following statement is correct to calculate the turnover number of an enzyme,
A) the enzyme concentration.
B) the initial velocity of the catalyzed reaction at [S] >> Km.
C) the initial velocity of the catalyzed reaction at low [S].
D) the Km for the substrate.
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#Question id: 13100
#SCPH06 I Botany
You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
You are using shotgun sequencing to determine the DNA sequence of the genome of this new bacterial species. For one strand of a 30-nucleotide long stretch of DNA, you get the following sequences out of your shotgun sequencing reaction. Assemble the entire 30-nt-long DNA sequence
5’-TGGGAGTTCCTCAAACGCGTTGTCACTGAC-3’
You put the DNA sequence that you have assembled into a computer program that tells you that the following piece of DNA, which comes from another bacterium, is a close match to the sequence you have sequenced from your bacterium: 5’-…TGGGCATTTCTCAAGCGGGTTGTAATGGAT…-3’
This 30-nt-long sequence fragment lies in the center of a gene, and that portion of the sequence encodes for this 10-amino acid-long part of a protein:
N-…Trp-Ala-Phe-Leu-Lys-Arg-Val-Val-Met-Asp…-C
You hypothesize that the sequence you have discovered is another bacterial species’ version of the same gene as this previously known gene. To measure how identical the two genes are at the DNA level and/or the two proteins are at the amino acid level, you can calculate a percentage of “identity” for each. This is the percent of nucleotides (for the gene) or the percent of amino acids (for the protein) that are identical between the two sequences.
What is the % identity between the two DNA sequences?
TLS Online TPP Program
#Question id: 14151
#I Life Science/ Life Sciences Group – I-V
Which of the following statement is true?
A) PAM matrices are derived from an evolutionary model whereas the BLOSUM matrices consist of entirely direct observations.
B) Because of BLOSUM matrices have less evolutionary meaning that is why the PAM matrices are used most often for reconstructing phylogenetic trees.
C) BLOSUM matrices are entirely derived from local sequence alignments of conserved sequence blocks, whereas the PAM matrix is based on the global alignment of full-length sequences.
D) BLOSUM matrices are more advantageous in searching databases and finding conserved domains in proteins.
TLS Online TPP Program
#Question id: 1276
#SCPH06 I Botany
Which of the following does not belong to the major families of cell surface adhesion molecules?
TLS Online TPP Program
#Question id: 11335
#SCPH28 | Zoology
Which of the following have minimum NET PRIMARY PRODUCTIVITY ?